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2008 Sep 02
2
Help with nonlinear regressional
Dear All, I am doing experiments in live plant tissue using a laser confocal microscope. The method is called "fluorescence recovery after photo-bleaching" (FRAP) and here follows a short summary: 1. Record/ measure fluorescence intensity in a defined, round region of interest (ROI, in this case a small spot) to determine the initial intensity value before the bleaching. This