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2007 Aug 14
0
Limma - 2x2 factorial design matrix
...of mutant at day 11 and day 21 alone? What is the logically theory behind to asign 1 or -1 to slides P21WT.P11WT for effect of Mt11 and Mt21? Does it has anything to do with the vector? Or we will subtract it with Wt at both time point in order for us to look at the effect of Mt11 and Mt21? I use Lucidea Universal ScoreCard as my spike in control. Do I need to flag them out before I carry out the analysis? Although I have include a Spot Type file, LimmaGUI still read all the spots as genes. Thank you Warm regards, Tee Sue Sean Department of Cell and Molecular Biology, Faculty of Biotechnolo...