search for: ligands

Hello everyone, I have biological data from a competition experiment where a free ligand is titrated against the binding of a protein. Now, I would like to fit a standard on-site binding curve to this data in order to obtain the IC50 and Kd values. Unfortunately I have not been able to find a package/function which allows such a fitting and calculates the results. Does anyone know if there is

...t R it isn't apparent to me what the problems are. Here is my code: png(imageFilename, width = 480, height = 360) par(cex = 0.75) plot(type = "s", y = control_Table, x = p_USER_Table, xlab = "% of compound database searched", ylab = "% of known ligands found", main = title , col = "#ff0000", pch = "20", xlim = (0.1, 100), ylim = (0, 100), log = "x") axTicks(side = 1, log = True) points(type = "s", y = control_Table, x = p_PMF_Table, col = "#00c000") points(type = &quot...

...it isn't apparent to me what the problems are. Here is my code: png(imageFilename, width = 480, height = 360) par(cex = 0.75) plot(type = "s", y = control_Table, x = p_USER_Table, xlab = "% of compou-10,nd database searched", ylab = "% of known ligands found", main = title , col = "#ff0000", pch = "20", xlim = (0.1, 100), ylim = (0, 100), log = "x") axTicks(side = 1, log = True) points(type = "s", y = control_Table, x = p_PMF_Table, col = "#00c000") points(type = &quot...

...be seen here: http://rcytoscape.systemsbiology.net/versions/current/gallery/TCGA/subnet.TCGA.02.0014.png This portrays 1) gene expression (green: under-expression; red: over-expression) 2) copy number (blue: high, black: low) 3) gene type (node shape: hexagons are kinases, arrows are ligands, diamonds are receptors, circles for everything else) 4) mutation status (not SNPs, but non-synonymous amino acid substitutions) 5) in the context of gene relationships, from KEGG A vignette for a larger version of this network is nearly complete. The Cytoscape network map is created from...