search for: imagenumb

Hi all, I have loaded a table called 'data' and used the split command to create a new table called 'datasplit' based on the variable 'a.ImageNumber' in 'data'. a.ImageNumber just specifies the image number that certain measurements are associated with (so there could be 330 measurements from image 1 and 297 measurements from image 2 etc). I would now like to retrieve information using the get command but I am running into some...

...;a" is nuclear measurements, "b" is cytoplasmic measurements a <- read.table(file.choose(), header=TRUE, sep=",") b <- read.table(file.choose(), header=TRUE, sep=",") #make a new data frame named "new" with important columns new <- data.frame(a$ImageNumber, a$AreaShape_Area, a $Intensity_IntegratedIntensity_OrigRFP, a $Intensity_IntegratedIntensity_OrigGFP, b $Intensity_MeanIntensity_OrigGFP) #make three new columns, "EstCytoIntensity" estimates Cytoplasmic intensity based on the mean measured intensity and an estimate of the nucl...

...es to the combined control data. However, there are a couple of 'wrinkles' to my problem: Not all the data is actually in sets of three. If there are no objects in a particular field,CellProfiler does not output anything for that image. For example, I could have the following data set: ImageNumber Measurement 1 5 2 7 3 8 4 3 6 9 So in this example, I would like to combine images 1 to 3 in one set and 4 to 6 in another set (image 5 was empty). Here is another 'wrinkle': For each image, there are multiple measurements based on the number of objects in the field of vi...