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Hello, I am trying to fit my Elisa results (absorbance readings) to a standard curve. To create the standard curve model, I performed a 4-parameter logistic fit using the 'drc' package (ExpectedConc~Absorbance). This gave me the following: > FourP A 'drc' model. Call: drm(formula = Response ~ Expected, data = SC, fct = LL.4()) Coefficients: b:(Intercept) c:(Intercept) d:(Intercept) e:(Intercept) 1.336 6.236 85.521 59.598 > summary(FourP) Model fitted: Log-logistic (ED50 as parameter) (4 par...

...ing similar) so we can test different solvents in making this standard curve. We currently use the drc package and this is our script for the 4 parameter: SC<-read.delim(file = "C:/Documents and Settings/rekem/My Documents/SCBook.txt", header = T, check.names = FALSE, as.is = TRUE) FourP<-drm(Response~Expected, data = SC, fct = LL.4()) plot(FourP, main = "LTB4 Standard Curve Zi Phase 7", xlab = "Expected (pg/mL)", ylab = "Response (%Bound)") Thanks for any help. Kevin McEnroy -- View this message in context: http://r.789695.n4.nabble.com/Adding-...