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Hi, I want to analyse DNA sequence data (mtDNA) in R as in calculate Fst, Heterozygosity and such summary statistics. Package Adagenet converts the DNA sequence into retaining only retaining the polymorphic sites and then calcuates Fst.. but is there any other way to do this? I mean analyse the DNA sequence as it is.. and calculate the statistics? Thanks! [[alternative HTML version deleted]]

Thank you for reading. I am trying to get sphericity values, and I understood I need to use mlm, but how do I implement a nested within subject design in mlm? I already read the R newsletter, fox chapter appendix, EZanova, and whatever I could find online. My original ANOVA anova(aov(resp ~ sucrose*citral, random =~1 | subject, data = p12bl, subset = exps==1)) Or anova(aov(resp ~