Displaying 1 result from an estimated 1 matches for "b635".
Did you mean:
635
2009 Mar 13
0
Singal channel spike in controls with custom microRNA slides - Normalization help needed
...tiher a
normalization by block per array or the mean of all the controls per array.
The gal file is also loaded with all the array structure. Each block
contains spike in controls.
Here is the code:
library(limma)
library(RColorBrewer)
library(vsn)
Cy5 <- "F635 Mean"
Cy5b <- "B635 Mean"
targets <- readTargets("targets.txt")
#My gpr files do only contain 1 channel (Cy5)
RG <- read.maimages(
targets$FileName,source="genepix",columns=list(R=Cy5,G=Cy5, Rb=Cy5b,
Gb=Cy5b))
RG$G <- NULL
RG$Gb <- NULL
RG$genes <- readGAL("array_human_mirs...