chziy429
2019-Nov-14 14:55 UTC
[R] Ask for help on the preprocessing of GEO microarray data with Oligo
Dear Sir I have downloaded the raw CEL data included in "GSE41418" from GEO and tried to process the raw microarray data according to the following Rscripts affydata <- ReadAffy(cdfname = "mouse4302mmentrezgcdf") eset <- oligo::rma(affydata) The raw data can be read by ReadAffy but failed in the normalization process. I have tried to process it with affy::rma, frma::frma and oligo::rma, but it still can not work. An error saying "address 0x7f854500a000, cause 'invalid permissions'" was returned. However this error message was not appeared in some other datasets like "GSE41342". I was wondering whether this file was corrupted. Could you please help me address this problem? Any suggestion and recommendation are welcome. Best [[alternative HTML version deleted]]
Bert Gunter
2019-Nov-14 17:36 UTC
[R] Ask for help on the preprocessing of GEO microarray data with Oligo
My recommendation is: Post on the BioConductor site, not here. Bert Gunter "The trouble with having an open mind is that people keep coming along and sticking things into it." -- Opus (aka Berkeley Breathed in his "Bloom County" comic strip ) On Thu, Nov 14, 2019 at 9:22 AM chziy429 <chziy429 at 163.com> wrote:> Dear Sir > > > > I have downloaded the raw CEL data included in "GSE41418" from GEO and > tried to process the raw microarray data according to the following Rscripts > > > affydata <- ReadAffy(cdfname = "mouse4302mmentrezgcdf") > eset <- oligo::rma(affydata) > > > > The raw data can be read by ReadAffy but failed in the normalization > process. I have tried to process it with affy::rma, frma::frma and > oligo::rma, but it still can not work. An error saying "address > 0x7f854500a000, cause 'invalid permissions'" was returned. However this > error message was not appeared in some other datasets like "GSE41342". I > was wondering whether this file was corrupted. > Could you please help me address this problem? Any suggestion and > recommendation are welcome. > > > > > > Best > [[alternative HTML version deleted]] > > ______________________________________________ > R-help at r-project.org mailing list -- To UNSUBSCRIBE and more, see > https://stat.ethz.ch/mailman/listinfo/r-help > PLEASE do read the posting guide > http://www.R-project.org/posting-guide.html > and provide commented, minimal, self-contained, reproducible code. >[[alternative HTML version deleted]]