Bogdan Tanasa
2019-Sep-11 20:14 UTC
[R] about paired or unpaired t.test or wilcox.test in R
Dear all, if would be great if you could please advise on the use of PAIRED or UNPAIRED T.TEST or WILCOX.TEST in R : let's say shall we have 2 samples : -- CONTROL : where we measure the expression of 100 genes G1 ... G100 in one million cells C1 ...C1mil -- TREATMENT : where we measure the expression of 100 genes G1 ... G100 in one million cells D1 ...D1mil when we compare the expression of these 100 genes G1 ...G100, in CONTROL vs TREATMENT, shall we use UNPAIRED TESTS, correct ? as the cells in CONTROL C1..C1mil are different than the cells in TREATMENT D1..D1mil ? thanks a lot ! -- bogdan [[alternative HTML version deleted]]
David Winsemius
2019-Sep-11 21:05 UTC
[R] about paired or unpaired t.test or wilcox.test in R
On 9/11/19 1:14 PM, Bogdan Tanasa wrote:> Dear all, > > if would be great if you could please advise on the use of PAIRED or > UNPAIRED T.TEST or WILCOX.TEST in R : > > let's say shall we have 2 samples : > > -- CONTROL : where we measure the expression of 100 genes G1 ... G100 in > one million cells C1 ...C1mil > > -- TREATMENT : where we measure the expression of 100 genes G1 ... G100 in > one million cells D1 ...D1mil > > when we compare the expression of these 100 genes G1 ...G100, in CONTROL vs > TREATMENT, shall we use UNPAIRED TESTS, correct ? > > as the cells in CONTROL C1..C1mil are different than the cells in TREATMENT > D1..D1mil ? thanks a lot ! > > -- bogdan > > [[alternative HTML version deleted]]????????? ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Rhelp is a plain text list. It is specifically for help with R coding difficulties, and it is specifically NOT to advice about chosing analysis plans. You question raises serious analysis issues regarding multiple comparisons and distributional assumption which I believe are still the subject of research. A more welcoming venue might be the BioConductor mailing list or CrossValitdated.com. -- David.> > ______________________________________________ > R-help at r-project.org mailing list -- To UNSUBSCRIBE and more, see > https://stat.ethz.ch/mailman/listinfo/r-help > PLEASE do read the posting guide http://www.R-project.org/posting-guide.html > and provide commented, minimal, self-contained, reproducible code.