Hi,I am having a problem with the ReadAffy() in the university R programming environment. I install affyPLM and invoke library(affyPLM) in the following way. I have the cel files in the craig subdirectory. Please let me know how to resolve the problem.Amit source("http://bioconductor.org/biocLite.R") biocLite("affyPLM") biocLite("gcrma") library(affyPLM)> setwd("c:/Users/amit.sengupta/Desktop/myRfolder/craig")> craig.data=ReadAffy()Error in AllButCelsForReadAffy(..., filenames = filenames, widget = widget,? : ? No cel filennames specified and no cel files in specified directory:c:/Users/amit.sengupta/Desktop/myRfolder/craig Included are the outputs of installations. - ? Home - ??Amit - ? Help Press ? for keyboard shortcuts. Close Ad - Mail - Contacts - Calendar - Notepad - Messenger - News Feed 1 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | - - - - - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | - | | | | | | | | preproc (5) People Amit Sengupta Hi David, After installing bioclite, I invoke library(affyplm) and run into this problem. > library(affyPLM) Loading required package: affy Error: package ?Biobase? required by ?affy? could no Sep 29 at 12:43 PM Wheeler, David Linnard Hello Sir, I had similar problems. For me I had to essentially resource bicolite everytime as below. Also, try to put quotations around your package names after the bioclite command, see it that works Sep 29 at 3:08 PM Hi David,This is the problem now with ReadAffy(). I have all files in myRfolder. Let me know what you think.Amit-------------------------------------------------------------> source("http://bioconductor.org/biocLite.R")Bioconductor version 3.1 (BiocInstaller 1.18.4), ?biocLite for help> biocLite("affyPLM")BioC_mirror: http://bioconductor.org Using Bioconductor version 3.1 (BiocInstaller 1.18.4), R version 3.2.0. Installing package(s) ?affyPLM? trying URL 'http://bioconductor.org/packages/3.1/bioc/bin/windows/contrib/3.2/affyPLM_1.44.0.zip' Content type 'application/zip' length 5289800 bytes (5.0 MB) downloaded 5.0 MB package ?affyPLM? successfully unpacked and MD5 sums checked The downloaded binary packages are in ??????? C:\Users\amit.sengupta\AppData\Local\Temp\RtmpITiCKT\downloaded_packages> biocLite("gcrma")BioC_mirror: http://bioconductor.org Using Bioconductor version 3.1 (BiocInstaller 1.18.4), R version 3.2.0. Installing package(s) ?gcrma? trying URL 'http://bioconductor.org/packages/3.1/bioc/bin/windows/contrib/3.2/gcrma_2.40.0.zip' Content type 'application/zip' length 490279 bytes (478 KB) downloaded 478 KB package ?gcrma? successfully unpacked and MD5 sums checked The downloaded binary packages are in ??????? C:\Users\amit.sengupta\AppData\Local\Temp\RtmpITiCKT\downloaded_packages> library(affyPLM)Loading required package: BiocGenerics Loading required package: parallel Attaching package: ?BiocGenerics? The following objects are masked from ?package:parallel?: ??? clusterApply, clusterApplyLB, clusterCall, clusterEvalQ, ??? clusterExport, clusterMap, parApply, parCapply, parLapply, ??? parLapplyLB, parRapply, parSapply, parSapplyLB The following object is masked from ?package:stats?: ??? xtabs The following objects are masked from ?package:base?: ??? anyDuplicated, append, as.data.frame, as.vector, cbind, colnames, ??? do.call, duplicated, eval, evalq, Filter, Find, get, intersect, ??? is.unsorted, lapply, Map, mapply, match, mget, order, paste, pmax, ??? pmax.int, pmin, pmin.int, Position, rank, rbind, Reduce, rep.int, ??? rownames, sapply, setdiff, sort, table, tapply, union, unique, ??? unlist, unsplit Loading required package: affy Loading required package: Biobase Welcome to Bioconductor ??? Vignettes contain introductory material; view with ??? 'browseVignettes()'. To cite Bioconductor, see ??? 'citation("Biobase")', and for packages 'citation("pkgname")'. Loading required package: gcrma Creating a generic function for ?nchar? from package ?base? in package ?S4Vectors? Loading required package: preprocessCore Warning message: package ?affy? was built under R version 3.2.1 [[alternative HTML version deleted]]