Luigi Marongiu
2015-Apr-13 22:00 UTC
[R] calculate Cy0 with qpcR package when fitting is failed
Dear all, i am trying to calculate the Cy0 from a series of PCR runs in the 384-well. I can create the regression model using the modlist function: ml<-modlist( obj, cyc = 1, model = l4, norm = "FALSE", remove = "none" ) where obj is the dataframe with the fluorescence data and ml is the resulting vector with 384 elements, but when i try to create the Cy0 values i get the error:> Cy0(ml, plot = "FALSE")Error in seq.default(MIN, MAX, by = DIVS) : 'from' cannot be NA, NaN or infinite I reckon that this is due to failed amplification data but i don't know how to screen the function from these abnormal values. when i run the modlist with arguments remove = "fit" i get the 372 elements but Cy0 returns the same error. How can i overcome this issue? Many thanks, Luigi