Hello, Below is some simple R code I have used to create a stacked bar chart. However the y-axis tick marks do not reflect the data. The minimum and maximum data values I have are 3073351 and 66425013 respectively but the graph has data from 0 and the maximum is different Data file is attached. What am I doing wrong? Please help as I cannot be sure the data is represented correctly in the graph (attached). fn <-"~/Documents/chr7Data.txt" x<-read.table(fn,check.names = TRUE, header=T) d <- data.frame(x) theChart<-ggplot(d, aes(x=factor(Congenic),y=Position, fill=Strain, theme_rect="white")) + geom_bar(stat='identity', position = "stack" ) + scale_fill_manual(values=c("deepskyblue1", "red2", "green3", "steelblue4", "lightblue4", "gray70")) + ylab(c("Position"))+ ggtitle("Strain specific SNP distribution in the congenics") + #coord_cartesian(ylim = c(min(d$Position), max(d$Position))) + #scale_y_continuous(breaks=seq(0, 200000000, 50000000)) + theme_bw()+ theme(plot.title = element_text(size=23), axis.text.x = element_text(size=13, angle=90), #20 before axis.text.y = element_text(size=13), panel.border = element_rect(colour="BLACK",size=0.5), axis.title.x = element_text(size=12), axis.title.y = element_text(size=12,angle = 90), panel.background = element_rect(fill="transparent"), legend.text=element_text(size=13), legend.title=element_text(size=12) #plot.background = element_rect(fill = "transparent",colour = NA) ) png("~/Documents/sendToRGrp.png",950,750) print(theChart) dev.off() Many thanks, Michelle This email may have a PROTECTIVE MARKING, for an explanation please see: http://www.mrc.ac.uk/About/Informationandstandards/Documentmarking/index.htm -------------- next part -------------- A non-text attachment was scrubbed... Name: sendToRGrp.png Type: image/png Size: 83252 bytes Desc: sendToRGrp.png URL: <https://stat.ethz.ch/pipermail/r-help/attachments/20150325/a01abb3d/attachment-0001.png> -------------- next part -------------- An embedded and charset-unspecified text was scrubbed... Name: chr7Data.txt URL: <https://stat.ethz.ch/pipermail/r-help/attachments/20150325/a01abb3d/attachment-0001.txt>