Dear All We are using the dendextend package to modify the plot output from a pvclust clustering The model is created with result <- pvclust(chord.1, method.dist="euclidian", method.hclust="average",nboot=100000) The plot is then formatted as following dend <- as.dendrogram(result) dend %>% pvclust_show_signif(result, signif_type = c("au"),alpha=0.05, signif_value = c(4, 1)) %>% set("labels_cex", c(1.5)) %>% set("labels_col", sp_col) %>% plot(horiz=TRUE, sub="", xlab="", ylab="",xlim=c(0.31, -0.17), ylim= c(3.5,93)) We are using the pvclust_show_signif function to set the line weight to '4' of pvclust branches with an alpha of less than 0.05. Is it possible to set multiple line weights for different alpha values e.g. lwd = 8 when alpha = 0.05 and lwd = 4 when alpha = 0.1? Best wishes Tom