Displaying 20 results from an estimated 500 matches similar to: "expression matrix"
2007 Sep 24
1
Error: cannot allocate vector of size...
Hi,
I want to change .RDA file to a text file. So I did as follows.
>load("my.rda")
>ls() ---> then it showed [1] exprs
>write.table(exprs,"C:\\my.txt",sep="\t")
I was successful with the first .RDA file. Then I used the same commands with
another .RDA file (172 MB)which is 4 times bigger than the first file (41.2 MB).
When I put the last command
2008 Mar 20
2
Error in function (classes, fdef, mtable): unable to find an inherited method for function "indexProbes", for signature "exprSet", "character"
Hello Everyone,
I am writing programs in R from 7 months and I am able to solve most of the
errors/issues except for this current post.
My Task is to read a Microsoft Excel file(textE_to_affy.csv) which contains
the Microarray Expression Values collected from the Illumina Microarray
experiment. These collected intensity values need to be normalized(Rank
Invariant Normalization) by using the R
2008 Feb 27
2
problem with creation of eSet
Hi,
I am having troubles with creating an eSet and would appreciate any help on
the following problem.
I am trying to create an eSet using the following code
pd <- read.table(file="pdata.txt",header =TRUE,row.names=1);
colnames(pd) <- c("type","tumor","time","id");
pdN <- list(type =
2006 Oct 12
1
getMethods() not finding all methods
Running R2.4.0 on Apple Mac OS X 10.4.8,
in Emacs ESS mode, and also R.app.
In an attempt to learn a bit more about
a particular method (geneNames in package affy)
I invoked
getMethods("geneNames")
which produced geneNames methods, but not the
one in affy (output below).
I had to know the signature (AffyBatch) in order
to find the method
> getMethod("geneNames",
2008 May 30
1
A question about *read.table()*
Hi list,
I have a question about using *read.table()* to read in a txt file.
Basically, it
consists of 16346 rows, 6 columns (no header). The code I used is:
exprSet <- read.table('process_all4_GSA2.txt', row.names = 1,header =FALSE)
and I got an error message:
> exprSet <- read.table('process_all4_GSA2.txt', row.names = 1,header
=FALSE)
Error in
2006 Oct 11
1
Possible bug in accessing methods documentation?
Hi,
Reading help("Documentation"), I'm led to believe that a help call
like:
?myFun(x, sqrt(wt))
Will search for help on the appropriate method in the case that myFun
is generic. This isn't working for me. Here is an example using the
Biobase package:
## If Biobase is not installed
source("http://bioconductor.org/biocLite.R")
biocLite("Biobase")
2003 Oct 27
1
initialization of S4 classes/methods
I'm seeing weird issues in methods initialization, i.e. loading
marrayClasses loads Biobase, and when explicitly done, as in
library(Biobase)
library(marrayClasses)
is fine, but when Biobase is loaded via a require statement in
marrayClasses' .First.Lib, I end up with:
Warning message: In the method signature for function "coerce", class
"exprSet" has no
2009 Jan 21
2
encountering difficulty asking R to manipulate the correct columns in Expression Set class (object 4). (PR#13464)
Full_Name: Guy W. Tillinghast
Version: 2.8.0
OS: Windows XP professional
Submission from: (NULL) (24.248.24.3)
I am encountering difficulty asking R to manipulate the correct columns in
Expression Set class (object 4).
I download the ALL data with:
library(golubEsets)
data(Golub_Merge)
Note, the data has the samples not in order. This is not R's fault (at least
not that I can tell):
>
2011 Oct 20
2
Creating affybatch objects from matrix (data from qPCR array)
Hi!
Is There a way to manually create an affybatch object from qPCR array data?
--
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Sent from the R help mailing list archive at Nabble.com.
2011 Nov 20
1
Deleting multiple rows from a data matrix based on exp value
Dear List,
I have a data matrix that consists of ~4500 rows and 25 columns (i.e.
an exprSet object that I converted via the 'exprs' function into a
data matrix)
Now I want to remove/delete the rows where all exp. values in that
particular row are below or equal to a specific cut-off value (e.g
1.11)
I have tried using several commands to address this issue:
>Matrix[rowSums(Matrix
2003 Dec 26
1
Problems converting output from Sweave to PDf
I am having trouble converting the output from Sweave
into a valid PDF file.
I have created a simple .Rnw file which will become a
full vignette at some point, but during the
intermediate testing, I got errors from texi2dvi.
This is what I have done.
0) Using a Windows Xp system
1) Created a file called GeneSpring.Rnw
2) Convert this to Tex using Sweave("GeneSpring.Rnw")
from within R
2007 Dec 20
1
custom subset method / handling columns selection as logic in '...' parameter
Dear R-helpers & bioconductor
Sorry for cross-posting, this concerns R-programming stuff applied on
Bioconductor context.
Also sorry for this long message, I try to be complete in my request.
I am trying to write a subset method for a specific class (ExpressionSet
from Bioconductor) allowing selection more flexible than "[" method .
The schema I am thinking for is the following:
2008 Feb 23
3
exprs function download
Hi,
I am supposed to use exprs as a function. Where can i download exprs
function? I tried searching at bioconductor and seach engine but no luck. Is
it located in one of the library in R?
thanks.
C
--
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Sent from the R help mailing list archive at Nabble.com.
2003 Mar 26
1
formal methods and classes and capitalization conventions
Martin Maechler has suggested that I post this comment to r-devel. It was
originally posted to bioconductor.
---------------------------------
I'd like to raise the issue of a capitalization convention for naming
objects in R. Almost everything in R used to be lowercase but recently
there is increasing use of mixed upper/lower case to define names. There is
potential for using the
2003 Sep 05
2
S4 Method Collisions with "[" (PR#4075)
Full_Name: Colin A. Smith
Version: 1.8.0
OS: Mac OS X 10.2.6
Submission from: (NULL) (216.102.90.18)
Both Biobase and my package annaffy use S4 classes to define methods for "[".
Both packages use the save image method of installation. (See annaffy 1.0.3 in
BioC CVS.)
Depending on how both packages are loaded, the Biobase definitions seem to be
getting masked out:
>
2011 Oct 07
1
Creating One Single Object with Phenotype and Expression Data
Hey,
I have code that can check the quality of a data set we're working with
(expression data), and I'm having some trouble writing code that would make
the expression data we have tie to other data we want to link it to (called
phenotype data). Does anyone have any advice on how I could make a single
object that would do this? Other relevant info: I want to use the pdata()
function,
2011 Oct 06
4
Mean(s) from values in different row?
Hello:
Is there a way to get a mean from values stored in different rows?
The data looks like this:
YEAR-1, JAN, FEB, ..., DEC
YEAR-2, JAN, FEB, ..., DEC
YEAR-3, JAN, FEB, ..., DEC
What I want is the mean(s) for just the consecutive winter months:
YEAR-1.DEC, YEAR-2.JAN, YEAR-2.FEB
YEAR-2.DEC, YEAR-3.JAN, YEAR-3.FEB
etc.
Thanks.
2006 Nov 18
1
Why SAM has totally diffent results in R2.1.1 and R2.4.0
Hi,
I am using SAM (from siggenes_1.2.11 package) method to select genes from
a microarray data set. After installing the latest R2.4.0 on my computer, to
my surprise the results are totally different from that calculated using
R2.1.1. Even the example code doesn't work the same way under these two
versions of R. Does anybody know what is going on? Thanks for any
suggestions.
2006 Feb 20
1
mva.pairs
Hello,
I am using the following code to plot an MVA plot.
library(affy)
library(Biobase)
library(limma)
library(gcrma)
pd<-read.phenoData("Clk.targets.2.txt",header=TRUE,
row.names=1,as.is=TRUE,sep="\t")
Data <- ReadAffy(filenames=pData(pd)$FileName,phenoData=pd)
Print(Data)
eset <- gcrma(Data)
write.exprs(eset,
2009 Oct 22
1
S4 object??
Hi all,
I have loaded the LIMMA and Biobase package and tried these commands:
library(limma)
library("Biobase")
data <- read.table("c:/temp/data.txt",header=T,row.names=1)
ExpressionData <- as.matrix(data[,c(2,3,4,6,7,8)])
eset <- new("ExpressionSet", exprs = ExpressionData)
design <- cbind(WT=1,P=c(0,1,1,0,1,1),G=c(0,1,0,0,1,0))
fit <-