similar to: limma - OneWayAnova

Dear list members, I am analysing my microarray data using limma package. Now I encounter several problems. Looking forward to your suggestions! Question 1: During the process of background correction using method="normexp", four warning messages appeared as "NaNs produced in: log(x)" (as you can see in the program posted below). What does that mean? How will it effect the

Dear all, I have a question about limmaGUI that is usually run in R environment. My problem is loading data into the programm. I have 6 gpr files that apparently are not compatible with limma. Everytime I'm trying to load the data (including a RNA targets file, an error appears:Error reading files. that I'm not sure,but seems to have something to do with the format of my files

Dear R-Help, I am using the function "topTable" from the LIMMA package. To estimate adjusted P-values there are several options (adjust="fdr" , adjust="BH") as shown below: topTable(fit, number = 10, adjust = "BH", fit$Name) I guess any of these options (fdr, BH, etc.) is using a default of FDR=0.05 which is quite conservative (i.e., very

Hello, I am a total beginner with ?R? and found a package ?venn? to create a venn diagram. The problem is, I cannot create the vectors required for the diagram. The manual say: "R> venn(accession, libname, main = "All samples") where accession was a vector containing the codes identifying the RNA sequences, and libname was a vector containing the codes identifying the

Dear All, I am using the LIMMA package to create 2 contrasts for my data and then calculating the vennCounts of the decideTests from the contrast.fit to be able to create venn Diagrams. The code works fine but the summary(results) shows zeros for all i.e. no gene were up regulated or downregulated. This is not true for my data since toptable output shows Log fold change greater than > 2. I am

Dear Sirs, How can I revert to an older limma version? Typing "install.packages("limma")" in R gives a list of mirrors. How can I install the version I want after I obtain and untar the file (e.g, limma_2.9.1.tar.gz)? I am running R 2.5.0 on a Linux machine (CentOS 5). When using limma it will not go past the read.maimages command. I get this error: Error in

Full_Name: Thomas Richardson Version: R 2.9.0 GUI 1.28 Tiger build 32-bit (5395) OS: 10.4.11 Submission from: (NULL) (216.254.15.72) I have encountered a problem with points in scatterplots disappearing in a quartz window when it is re-sized (to make it larger). I am constructing an 8x12 matrix of scatterplots each containing approx 600 points. In order to get them in the window I remove the

Hi everyone, I'm trying to generate an RNA degradation plot of the Estrogen example data plot, but seem to get an error. I've tried defining an ylim value, ylim=c(0,30) , but it doesn't seem to work either. My code is as follows: > RNAdeg<-AffyRNAdeg(Data) > png(DegLoc, width=720, height=720) > par(ann=FALSE) > par(mar=c(3,3,0.1,0.1)) >

It might have a noticeable effect on clients. I encountered (probably triggered by this in some way?) that I was unable to het the 'read' bit set in macOS Mail.app. Maybe (as I am doing HA with round robin) the Mail.app client got to one dovecot repository on one tcp connection and then on the other. Is there a reason why syncing tis move from new to cur is a bad idea? Gerben Wierda

Dear mailing group, This is my first time here. Glad to have this resource! I am currently trying to load an Excel file into R (limma package loaded) using the source(*name of directory*) command, but it cannot open the file. I renamed the file as .R and .RData, to no avail. The Excel data contains one gene name per row and about 100 data points per gene (columns). I am only used to

Hi, this is a problem that occurs in the presence of two libraries (limma, xlsx) and leads to a crash of R. The problematic code is the wrong application of sweep or the product ("*") function on an LIMMA MAList object. To my knowledge, limma does not define a "*" method for MAList objects. If only LIMMA is loaded but not package xlsx, the code does not crash but rather

On January 6, 2023 3:56:39 AM GMT+02:00, Gerben Wierda <gerben.wierda at rna.nl> wrote: >One step further in my quest to create a replacement mail server. > >I now have my old mail server (2.3.19.1, macOS + MacPorts) and my new (2.3.20, Alpine Linux, Docker, apk package). When I turn on replication it works, but, after a while I see: > >Jan 06 00:50:31 replicator: Panic: data

Dear All, How to cite the PDF user's guide for the LIMMA package? This is not about how to cite the LIMMA package. Roger Roger L. Vallejo, Ph.D. Computational Biologist & Geneticist U.S. Department of Agriculture, ARS National Center for Cool & Cold Water Aquaculture 11861 Leetown Road Kearneysville, WV 25430 Voice: (304) 724-8340 Ext. 2141 Email: roger.vallejo@ars.usda.gov

Hi, I am following the protocol outlined here for analysis of single channel Agilent microarray data: http://matticklab.com/index.php?title=Single_channel_analysis_of_Agilent_microarray_data_with_Limma I keep getting the following error message when using Limma's read.maimages function to load my data into an RGList object: Error in RG[[a]][, i] <- obj[, columns[[a]]] : number of

Hi all. I've got a really dumb question for anyone. How do I write the output of a limma analysis (basically the topTable) to a text file? I want to output the topTable for the entire microarray (not really a topTable anymore I suppose..). Thanks for any advice! -Josh

Hi, I have two data set of normalized Affymetrix CEL files, wild type vs Control type.(each set have further three replicates). > wild.fish AffyBatch object size of arrays=712x712 features (10 kb) cdf=Zebrafish (15617 affyids) number of samples=3 number of genes=15617 annotation=zebrafish notes= > Dicer.fish AffyBatch object size of arrays=712x712 features (10 kb) cdf=Zebrafish (15617

Hi Hilmar, weird. The memory problem seems be due to recursion (my R, version 3.3.3, says: Error: evaluation nested too deeply: infinite recursion / options(expressions=)?, just write traceback() to see how it happens), but why does it segfault with xlsx? Nb xlsx is the culprit: neither rJava nor xlsxjars cause the problem. On the other hand, quick googling for r+xlsx+segfault returns tons of

Hi Everyone, I have been trying to read some Arrayvision files( 2 channel cDNA) and am having some problem. My code is : setwd('C:/work/data/limma/ndd1'); files <- c('ndd1_1.txt','ndd1_2.txt','ndd1_3.txt'); RG=read.maimages(files,"arrayvision",sep="\t"); #Normalisation MA=normalizeWithinArrays(RG); #plotPrintTipLoess(MA); #Fit Linear

Hi, I have a question concerning the analysis of some affymetrix chips. I downloaded some of the data from GEO GSE11324 (see below). In doing so I'm stuck after I identified the probesets with significant changes. I have problems in assigning probeset specific gene names as well as getting the genomic coordinates. Furthermore I have no clue how to deal with the fact, that most genes have

I can confirm this in a slightly different setting, but still using two-way sync between two dovecots. On e is 2.3.19.1 running on macOS Monterey, the other is 2.3.20 running in an alpine container on Ubuntu. Gerben Wierda (LinkedIn <https://www.linkedin.com/in/gerbenwierda>) R&A IT Strategy <https://ea.rna.nl/> (main site) Book: Chess and the Art of Enterprise?Architecture