search for: miraibio

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2011 Feb 25
0
creating standard curves for ELISA analysis
...I am a technical support for Hitachi Solutions and I saw your post regarding ELISA analysis. When I opened up your .csv file, I noticed it was a Luminex file from the Data Collector 1.7 software. My company developed a multiplex data analysis software for the Luminex platform called http://www.miraibio.com/masterplex-qt/qt-luminex-quantitative-data-analysis-software.html MasterPlex QT . I used this software and did ran the curve-fitting for you and included both the report (.csv file) with the final results and the MasterPlex QT project file (.mlxq). Here is one of your standard curves for &quo...
2011 Feb 01
4
Fitting ELISA measurements "unknowns" to 4 parameter logistic model
Hello, I am trying to fit my Elisa results (absorbance readings) to a standard curve. To create the standard curve model, I performed a 4-parameter logistic fit using the 'drc' package (ExpectedConc~Absorbance). This gave me the following: > FourP A 'drc' model. Call: drm(formula = Response ~ Expected, data = SC, fct = LL.4()) Coefficients: b:(Intercept) c:(Intercept)