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Hi, is it possible to merge two data frames while preserving the row names of the bigger data frame? I have two data frames which i would like to combine. While doing so I always loose the row names. When I try to append this, I get the error message, that I have non-unique names. This although I used unique command on the data frame where the double inputs supposedly are thanks for the help

Hello, I'm trying to paste two columns of a matrix GB_ACC GENE GENE_SYMBOL NM_010657 NM_010657 NM_027162 NM_027162 NM_198863 NM_198863 NM_010727 NM_010727 These two column are a part of a 41,000 x 26 matrix. I wanted these two column to be pasted into one (the firstone, GB_ACC). I tried to do it with a for loop, but it takes ages to finish: for( i in

Hi, I would like to substitute a semicolon with two double quotation marks and a comma inbetween. It suppose to look like that: I have: FBpp0070086;FBpp0099643;FBpp0112915 I would like to have: "FBpp0070086","FBpp0099643","FBpp0112915" I tried with various numbers of backslashes, but noe have worked. for example: gsub(";", "\\\",\"",

Hello, I have two tab-delimited files which I would like to combine. In the first one I have gene IDs (Unique) on column 1 and than various experimental results from microarray analysis (see attached files list1 ) the second arrays have the same genes IDs (more and in a different order, some are double) (see attached files list2 ) What I would like to do is to search in the second list for gene

Hello R users, I'm trying to extract random samples from a big array I have. I have a data frame of over 40k lines and would like to produce around 50 random sample of around 200 lines each from this array. this is the matrix ID xxx_1c xxx__2c xxx__3c xxx__4c xxx__5T xxx__6T xxx__7T xxx__8T yyy_1c yyy_1c _2c 1 A_512 2.150295 2.681759 2.177138 2.142790 2.115344 2.013047

Hi BioC user, I have a problem extracting the gene set I would like to work with. Here is I work with my data: normData <- read.delim("normalizedData.txt",sep ="\t") ######### two class unpaired comparison # y must take values 1,2 classes <- c(-1,-2,1,2) #prepere the data for the samr analysis data.x <-as.matrix(normData[,8:11]) d=list(x=data.x,y=classes,

Hello everybody, I have a a matrix of 2 columns and over 27k rows. some of the rows are double , so I tried to remove them with the command unique(): > Workbook5 <- read.delim(file = "Workbook5.txt") > dim(Workbook5) [1] 27748 2 > Workbook5 <- unique(Workbook5) > dim(Workbook5) [1] 20101 2 it removed a lot of line, but unfortunately not all of them. I wanted

Hi, at the moment I'm trying to understand the limma/affy Packages. I've tried every example I could find to try and understand the ways to create design and contrast matrices. I must admit though, that I can't figure it out. I did a few times the estrogen files from the book "bioinformatics and computational biology solutions using R and Bioconductor". I know it's a

Hallo, I have a set of S4 and S3 classes together in one script. While running this script I create a lot of new functions and objects An example for S3 and S4 classes: ## S3 classes pt <- list(x=1,y=2) class(pt) <- "xypoint" xpos <- function(x, ...) UseMethod("xpos") xpos.xypoint <- function(x) x$x ypos <- function(x, ...) UseMethod("ypos")

Hey, As a beginner to the limma package I tried to understand how to define a design matrix. I did both experiment examples in the "Limma user's Guide" manual. Afterwards I wanted to do it with my own data. Here I encounter a few problems. As an example I used four Control samples and 4 treated samples. 1. This is how my target files looks like: filename treated time.h

Hi all, I'm trying my best in changing the names of my experiments in the spectral map. My experiments are from six different time points, two as control and two treated experiments. I set have for each time point a different color . Now I want to change the symbols so that I have for the treated experiments one and for the controls a different one. But I want only two different symbols

Hello, I was wondering whether there is a possobility to add File types to the list of filters known by R. I'm using the function choose.files in Windows. I have some files I always use and i would like to filter my option to these kind of files. I have some "atr", "cdt", "gct", "gmt" etc. Is there a way of telling R to filter only these files?

Hi everybody, I have a problem when trying to do the quality control with the packages simpleaffy and affyQCReport with the drosophila chip 2.0 At first I got the messeage, that the *.qcdef file is not there. I followed the instructions in tha manual and created the file like that: array drosophila2cdf alpha1 0.05 alpha2 0.065 spk bioB AFFX-r2-Ec-bioB-3_at spk bioC AFFX-r2-Ec-bioC-3_at spk bioD

Hello, Is it possible to change the default repositories? I've already changed the list in the repositories file under etc/ this is how my repositories file under R/etc looks like: row name menu_name URL default source win.binary mac.binary CRAN CRAN @CRAN@ TRUE TRUE TRUE TRUE BioC Bioconductor http://www.bioconductor.org TRUE TRUE TRUE FALSE

Hello everyone, I have a matrix of over 40000 line and about 30 columns. For my analysis I would like to add another column with ascending numbers (column header should be "order", and than 1,2,3,4 ....the end of the matrix). During my analysis I reorder them ( due to merge commands by a different column). How do I add such a column in an ascending order (or descending for what it

Hi everybody, I am having a problem building a ROC curve with my data using the ROCR package. I have 10 lists of proteins such as attached (proteinlist.xls). each of the lists was calculated with a different p-value. The goal is to find the optimal p-value for the highest number of true positives as well as lowaest number of false positives. As far as I understood the explanations from the

Hello all R users, I have a problems transforming (or maybe better regrouping) a data.frame. I have a big data.frame, which I would like to sum up according to a specific column. This is an example of my matrix: ID gen 0042787 gen2 0016070 gen2 0016070 gen3 0007409 Gen1 0007409 gen3 0006511 gen2 0006417 gen3 0016070 gen4 0006511 gen4 I want to rearrange the matrix

dear all, I have a problem with a masked object in a package we created here. we make a package for a workflow of internal analysis of microarray data. to create the package we used: > install.packages(pkgs="affyAnalysis", repos=NULL) > R CMD INSTALL affyAnalysis Erzeuge Verzeichnisse ... Erzeuge DESCRIPTION ... Erzeuge NAMESPACE ... Erzeuge Read-and-delete-me ... Kopiere

Hello everybody, I'm trying to install a package I have built. This package contains three scripts with various functions (S3 as well as S4 classes) I run at first the package.skeleton command with: > package.skeleton("affyAnalysis", namespace=TRUE, code_files =c("defS3.R", "defS4.R", "qc.R")) affyAnalysis is the name of the supposed package and

Hi, I have a problem on my mac when trying in R to produce png images. I am getting this warnings with the ArrayQualityMetrics package: > arrayQualityMetrics(rma_fatBody, outdir="normData", force =T) The report will be written into directory 'normData'. KernSmooth 2.23 loaded Copyright M. P. Wand 1997-2009 (loaded the KernSmooth namespace) libpng warning: Application built